Fossil DNA

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Fossil DNA

Also known as... ancient DNA. The New Shorter Oxford Dictionary offers the precise explanation of ‘ancient’ as ‘belonging or pertaining to the period before the fall of the Western Roman Empire in AD 476.’ DNA can be recovered from fossils much older than 1500 years, but the inevitable time-dependent decay of the DNA structure due to oxidation and hydrolysis makes retrieval increasingly difficu...

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Fossil avian eggshell preserves ancient DNA.

Owing to exceptional biomolecule preservation, fossil avian eggshell has been used extensively in geochronology and palaeodietary studies. Here, we show, to our knowledge, for the first time that fossil eggshell is a previously unrecognized source of ancient DNA (aDNA). We describe the successful isolation and amplification of DNA from fossil eggshell up to 19 ka old. aDNA was successfully char...

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Ultrastructure and DNA Sequence Analysis of Single Fossil Sediment Cells

The method we have developed allows the cytoplasm of a single fossil cell to be sufficiently preserved as to be distinguished by fluorescence microscopy. Analysis of fossil DNA involved PCR amplification of the spacer region between rrn5 and trnR genes in the chloroplast genome. The DNA and consensus sequences, obtained from the same PCR amplification fragment, were analysed by BLAST search and...

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Bacillus DNA in fossil bees: an ancient symbiosis?

We report here the isolation of DNA from abdominal tissue of four extinct stingless bees (Proplebeia dominicana) in Dominican amber, PCR amplification of a 546-bp fragment of the 16S rRNA gene from Bacillus spp., and their corresponding nucleotide sequences. These sequences were used in basic local alignment search tool searches of nonredundant nucleic acid data bases, and the highest scores we...

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Fossil embryos deep in the fossil record

Embryos have been found in ‘old’ rocks on four continents, especially in Siberia, Nevada, Australia and China. They have been discovered in sedimentary rocks of even Late Neoproterozoic age, assuming the evolutionary geological column1 (figure 1). The Neoproterozoic is the period, so called, between 1 billion and 550 million years ago. This discovery was in the Doushantuo Formation, Guizhou Pro...

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ژورنال

عنوان ژورنال: Current Biology

سال: 2000

ISSN: 0960-9822

DOI: 10.1016/s0960-9822(00)00668-0